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Antibodies certain to the blocking peptide now not bind on the epitope over the target protein. By evaluating the staining from the blocked antibody vs . the antibody by yourself, one can see which staining is specific.
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This guide outlines even more feasible optimization of mobile and immunoassay parameters to ensure the absolute best results are...
This information outlines further more achievable optimization of mobile and immunoassay parameters to ensure the best possible effects are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind exclusively on the goal antibody and block antibody binding. These peptide typically includes the epitope acknowledged from the antibody.
Target Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S section.
Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in endorsing cell-cycle development within the absence of website pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two more splice variants have been reported but thorough nucleotide sequence information and facts just isn't still accessible.
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